A light-microscopy-based technology for tissue reconstruction would enable observation of structural dynamics in living systems. To address how this crowded and complex tissue’s architecture, connectivity and functional activity evolve over time, one would ideally employ a technology that enables imaging and in silico reconstructing living brain tissue.Įlectron microscopy (EM) reconstruction offers the most detailed insights into brain architecture by tracing all neuronal structures and determining connectivity with single-synapse accuracy, thus unraveling ‘connectomes’ 2, 3, 4, 5, 6, 7, 8, 9, 10 however, this is limited to static representations, whereas specific molecular labeling requires correlative approaches 11. LIONESS opens up avenues for studying the dynamic functional (nano-)architecture of living brain tissue.īrain computation and information storage are intimately linked to the structure of a synaptic network of ~86 billion neurons 1 in humans. This allows dense deep-learning-based instance segmentation and 3D reconstruction at a synapse level, incorporating molecular, activity and morphodynamic information. This leverages optical modifications to stimulated emission depletion microscopy in comprehensively, extracellularly labeled tissue and previous information on sample structure via machine learning to simultaneously achieve isotropic super-resolution, high signal-to-noise ratio and compatibility with living tissue. Here we solved these challenges by developing an integrated optical/machine-learning technology, LIONESS (live information-optimized nanoscopy enabling saturated segmentation). And that would be a disciplinary offense.Three-dimensional (3D) reconstruction of living brain tissue down to an individual synapse level would create opportunities for decoding the dynamics and structure–function relationships of the brain’s complex and dense information processing network however, this has been hindered by insufficient 3D resolution, inadequate signal-to-noise ratio and prohibitive light burden in optical imaging, whereas electron microscopy is inherently static. Of course, you can’t tell anyone anything, because that would be a breach of the Official Secrets Act 1911, 1920, 1989. The few survivors from the first police and army units thrown in. Quite literally, in some cases, of course.Īnd now, there is just you. The screams of “its got a bloody chainsaw!” over the radios probably didn’t do much for morale, as whole units were chewed up. The first army units hadn’t been warned what to expect. The police were sent in, but that wasn’t even a challenge for the specimens. Others are shambling amok or even jumping amok, but you get the idea. No-one was left alive to turn off the specimen-cloning equipment. They had just got on to the chain gun and rockets when the government tried to secretly shut down their secret program.īut, in the typical way these things go, the program didn’t want to be shut down. The bigger ones were the first ones they tried arming. The basic ones will just munch on your arm and try to disembowel you. They are the left-over “specimens” from a cheap and dirty government program to clone soldier-monsters. The aim – cleanse each area of zombies, in waves, until you get to the last one. Up to 6 players in online co-op mode, or just you, on your own, playing the Solo mode. In 2013 a new "Objective Mode" was added to the game. After the Linux version launched, all non-Steam servers were closed and the game became Steam exclusive. Killing Floor 2 was announced on May 8th 2014. Killing Floor was initially released as an Unreal Tournament 2004 total conversion mod in 2005 and was later released as a retail version on May 14th, 2009 for Windows, May 5th 2010 for Mac OS X and Linux in November of 2012.
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In 2001 he appeared as Finch again in “American Pie 2”, meanwhile also cast in the significant part of Mike Platt, in 28 episodes of “Off Centre”, through 2002. Thomas entered the year 2000 with 13 on-screen and numerous theater appearances under his belt, landing the role of Russell in seven episodes of “Brutally Normal” right then, as well as the recurring role of Gary in the prodigy series “The X-Files”, in which he appeared again, and two other roles. That year he also played four other roles, one of which marked his second appearance in “Law & Order.” He landed his first film role in 1996, as Young Flaco in “Illtown”, aside from also showing up in an episode of “Law & Order”, then in 1997, he appeared alongside legendary Natalie Portman in a stage adaptation of “The Diary of Anne Frank.” After another three TV roles in 1998 and one more a year later, he landed his most significant role thus far – Paul Finch in the four-film installments of world-renowned “American Pie”, the first of which premiered in 1999. Thomas’ onscreen breakthrough came when he was 14, thanks to the role of Dave in an episode of the romantic TV series entitled “One Life to Live” in 1994, as well as Matt Dorney in “Are You Afraid of the Dark?” He played a role in Brooklyn Shakespeare Company’s “Richard III” before he was 10 years old, and debuted on Broadway in “Four Baboons Adoring The Sun” in John Guare’s Lincoln Center Production as an understudy at age 12 in 1992, which marks his career beginning. He matriculated from New York’s Professional Children’s School, a preparatory dramatic arts college, in 1998, at which point his acting career was already well underway, thus he never attended college.īy the time Eddie’s career officially started, he had already made an enviable number of appearances across various theaters. Eddie grew up with a strong interest in acting, participating in school plays as early as age seven. The name he goes by today was adopted at the start of his acting career, leaving his birth name unknown. Kaye was raised apparently an only child in his birthplace, by Jewish parents, specifically in New Dorp neighborhood, right across from a train station. Each of the Premier category hotels comes with complimentary Universal Express Unlimited passes for your entire party staying at the hotel.Īll Universal Orlando hotels provide complimentary transportation to and from its theme parks and Early Park Admission, which allows your party to get into the theme parks one hour before the regularly scheduled opening time. Its sister property, Dockside Inn and Suites, will offer another affordable option when it opens.įor a more luxurious hotel experience, Universal Orlando offers three Premier category hotels including Loews Portofino Bay Hotel, Hard Rock Hotel at Universal Orlando and Loews Royal Pacific Resort. Universal Orlando has launched its first-ever Value category hotel: Universal’s Endless Summer Resort – Surfside Inn and Suites, with room rates beginning around $100. If you are looking for a budget-friendly hotel, you are in luck. You can choose a hotel from eight options that include standard rooms and a multitude of suites to accommodate every style, size and budget. What better way to relieve some stress than by planning everything out and being able to control all aspects of the vacation? With all the details that need to be accounted for, researching different resorts and building an itinerary from scratch can be daunting.Make your Florida vacation one to remember with a stay at this hotel and theme park complex.Ī visit to Universal Orlando Resort offers a memorable vacation for all the family, with accommodation, entertainment and theme park thrills all in one place.
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Rosario put the Gators ahead for good with 5:29 remaining, draining a 3-pointer for a 53-52 lead. The Rebels scored only eight points in the first 12 minutes of the second half. While Florida was heating up, shooting 52 percent in the second half, the Ole Miss offense was grinding to a halt and the 3-point shooting reverted to previous form. Young's dunk gave the Gators a 48-46 lead - their first since the opening minutes. Williams hit four of the team's six 3-pointers.īut Florida slowly climbed back into the game in the second half behind Young and some well-timed 3-pointers, including Scottie Wilbekin's with 10:11 remaining that tied the game at 46. They made 14 of 23 shots from the field in the first half, and had an uncharacteristic good touch from long range. The Rebels went on a 14-0 run to take a 20-4 lead in the first 8 minutes before settling for the 10-point halftime lead. That negated the Gators' one major strength - superior guard play - and they struggled to find open 3-point looks for much of the game. Ole Miss got the pace it wanted from the very beginning, forcing Florida into a half-court, physical game. They couldn't duplicate that success in the second half, making just 8 of 29 (27.6 percent) from the field. The Rebels shot 60.9 percent from the field in the first half. Terrance Henry had 21 points and 10 rebounds for Mississippi (13-7, 3-3), while Nick Williams added 14 points. "I never thought they got rattled or overwhelmed." "I thought that our guys stayed the course," Florida coach Billy Donovan said. Erving Walker had 10 points and nine assists. Young was 7 of 10 from the field off the bench despite playing with tendinitis in his right ankle. But the Gators worked their way back thanks to lockdown defense and clutch 3-pointers from Boynton and Mike Rosario. In the second half we guarded the 3-point line a little better, but really, it was just kind of the law of averages."īoynton admitted keeping cool was tough when Ole Miss was draining 3-pointers in the first half - hitting all six of its attempts from long range in front of a raucous crowd at Tad Smith Coliseum.įlorida (16-4, 4-1 Southeastern Conference) fell behind 20-4 in the opening minutes and trailed 38-28 at halftime. They hit tough shots and were just killing it. "We were playing defense but they hit shots. "The best thing for us was we never panicked," Boynton said. Patric Young scored 15 points and Boynton added 12 as Florida won for the sixth time in seven games. The 14th-ranked Gators clawed back from a double-digit first-half deficit to beat Mississippi 64-60 on Thursday night. The synopsis: The Rebels play defense, but can't shoot. Florida's Kenny Boynton had read his scouting report on Mississippi. Simply call the number on the front of your service contract. 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The bottom of the chicken should be golden brown at this point. You’ll start by heating a drizzle of olive oil in the pan over medium heat. It allows the chicken to brown on the bottom without any chance of sticking, tearing, or drying out. You will want to use a nonstick skillet for this recipe (this one is my absolute favorite, pictured). You can go simple with salt and pepper, or I always like to add garlic powder, onion powder, smoked paprika and oregano, no matter what I’m using the chicken for. Next, season the chicken with desired seasonings. Here’s how to make shredded chicken with step-by-step photos using my favorite stovetop method (full recipes of all methods in the printable recipe card at the bottom of the post): season THE CHICKENįirst, pat the chicken dry with paper towels so the seasonings stick and to ensure a beautiful sear. This creates a half poach/steam method, which cooks the chicken gently, without losing any moisture, creating incredibly juicy chicken. After the chicken is seared on the first side, it’s flipped, and allowed to sear on the second side along with chicken broth. This golden crust is the result of the Maillard reaction, also known as the flavor reaction, in which amino acids and reducing sugars produce browning and complex flavor that can’t be achieved any other way. The chicken first sears on one, creating a crust of flavor that seals in the juices and keeps your chicken tender while cooking. Pick whichever cooking method works best for you! However, the stovetop method is my absolute favorite for the most flavorful shredded chicken. There are three easy ways to make shredded chicken: on the stovetop, in the slow cooker, and in the Instant Pot (or pressure cooker). What’s the best way to make shredded chicken? A tropical storm becomes a category one hurricane when winds reach sustained speeds of 74 miles per hour. 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They occur in tropical regions and are called typhoons in South-East Asia and the Pacific, and cyclones in the Indian Ocean. Hurricanes are characterised by a calm central region of low pressure between 12 to 60 miles in diameter, known as the eye. The term hurricane is derived from Hurican, the name of a native American storm god. How hurricanes are bornĪ hurricane is a fiercely powerful, rotating form of tropical storm that can be 124 to 1240 miles in diameter. Here, New Scientist explains how hurricanes form and why Hurricane Katrina was so destructive. The cost of the damage may top $100 billion. Thousands have been killed or injured and more than half a million people have been displaced in a humanitarian crisis of a scale not seen in the US since the great depression. Katrina has left a disaster zone of 90,000 square miles in its wake – almost the size of the UK. 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